李芳仁

李芳仁 Ph.D. Fang-Jen Lee

特聘教授 Distinguished Professor

研究領域：
蛋白質表現與囊胞傳遞的調控研究
Regulation of Protein Trafficking

研 究 室：
臺大醫院東址2樓分子醫學所
Institute of Molecular Medicine, 2nd Floor, Main Building(NTUH East Campus)

Tel：886-2-23123456 ext. 265730


　〈細胞生命的重要基礎：囊泡運輸研究〉

  1. Mechanisms regulating membrane and vesicle trafficking

My long-standing research interest is to dissect the molecular mechanism involved in membrane and vesicle trafficking. My lab has been using yeast as a model organism to investigate the roles of small GTPases ADP-ribosylation factor (Arf)-like proteins (Arl) in regulating vesicular trafficking. We show that Arl1 and Arl3 play roles in trans-Golgi-mediated vesicular trafficking. We also demonstrate that ER stress mediates Arl1 activation to regulate vesicular transport at the Golgi. We will further explore the mechanisms of Arl1 and Arl3 in regulating vesicular traffic under the cellular stresses.

  1. Mechanism underlying Arf3-mediated yeast invasive growth

Many fungi will switch to filamentous growth to search for nutrients in energy-depleted environments. We have characterized the role of Arf3 in yeast invasive growth. A novel spatial activation of Arf3 plays a role in regulating Bud2 activation during glucose depletion-induced invasive growth. We identified a novel GEF Snf1/AMPK, which is responsible for Arf3 activation in response to glucose depletion. We also found that mammalian Arf6 and AMPK function together in cell migration and invasion. Understanding the regulatory pathway underlying the mechanism of yeast Arf3-, mammalian Arf6, and Snf1/AMPK-mediated invasive growth may facilitate new drug development in the future.

  1. Mechanisms of Arl4 small GTPases in regulating membrane and cytoskeletal dynamics

We identified mammalian Arl4A and Arl4D and showed that Arl4D expression has tissue or differentiation specificity. We demonstrate that Arl4A is distributed in the nucleus and nucleolus, and is associated with early development, somite formation, and central nervous system differentiation. Arl4 subfamily proteins are associated with initiation and progression of many cancers. Activation of Arl4 facilitates cell morphogenesis, decreases actin filaments, and promotes cell migration. Hundreds of hereditary diseases, autoimmune diseases, and many cancers are known to be caused by the blocking of certain molecules transport in the cell. This project provides a platform to integrate basic research in Arl4 GTPases with clinical application of identifying cancer biomarkers and potential drug target. We will explore how Arl4 proteins be regulated to exert their function in membrane and cytoskeletal dynamics, thereby modulating cell migration, adhesion, invasion, and proliferation.

  • 1976-1980 B.S. National Taiwan University, Taipei, Taiwan, R.O.C.
  • 1982-1984 M.S. North Carolina State University, Raleigh, N.C.
  • 1984-1986 Ph.D. North Carolina State University, Raleigh, N.C.

  • 1987-1990
    Postdoctoral Fellow in Department of Genetics, Harvard Medical School, and Molecular Biology Fellow in Department of Molecular Biology, Massachusetts General Hospital, Boston, M.A.
  • 1990-1994
    Senior Staff Fellow in Laboratory of Cellular Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD.
  • 1994- 2001
    Associate Professor in Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 2001- 2007
    Deputy Director in Department of Medical Research, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 2001- 2006
    Professor in Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 2004 - 2010
    Director in Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 2006- present
    Distinguished Professor in Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 2009- 2012
    Associate Dean for Research Affairs, and Director for Branch Office of Research and Development, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 2009- 2012
    Director for Translational Medicine Ph.D. Program, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 2011- 2012
    Associate Dean for Research and Development, National Taiwan University, Taipei, Taiwan.
  • 2012- 2013
    Acting Director for Institute of Brain and Mind Science, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 2012- 2019 
    Vice President and Dean for Research and Development, National Taiwan University, Taipei, Taiwan.
  • 2013 - 2019 
    Director in Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 2018 - 2021 
    President, Taiwan Society of Biochemistry and Molecular Biology.

 

博士班：蔡佩娟、張庭瑋

碩士班：柯品琪、劉晏甄、林佳盈

博士後研究：邱婉筠

研究助理：陳迦燈、蔡佳穎、陳孜耘、簡士宏

Alumni:(博士班)：
林靜宜 （美國克里夫南醫院神經科學研究部研究員）
卜樂妙 （國立高雄師範大學生物科技系副教授）
黃純芳 （華安醫學股份有限公司科學研究處處長）
陳宜君 （台大醫學院內科教授）
詹琍婷 （台大醫學院研發分處資深高級研究技師）
劉雅雯 （台大分子醫學研究所教授）
李純純 （成功大學生科糸副教授）
陳冠毓 （國家衛生研究院科管處研究員）
蔡佩秦 （台灣泰福生技公司研究員兼研發組長）
張翎君 （美國Memorial Sloan Kettering Cancer Center研究員）
許家維 （台灣大學生化科學研究所助理教授）
江采蓁（台大醫學院外科博士後研究員）
陳冠融（三顧生醫研究員）
林新晉 （浩鼎生醫研究員）
林明潔 （美國哈佛大學醫學院博士後研究員）
邱婉筠（台大醫學院分子醫學研究所博士後研究員）

Alumni:(博士後研究): 游佳融、林玉俊、洪嘉穗、杜思薇、張玉雲、蔡岳佐、蔣采昕、陳柏翰

Alumni:(碩士班): 余維倫、董璐、郭哲成、黃凱彬、朱之文、張逸蘋、林亞慧、李思緯、吳宗聖、方瓊瑩、黃小娟、蔡松蒲、林靜慈、陳吳姍、鄭涵聲、何瑞芝、蔡岳佐、劉宜昀、朱穎潔、許俽嘉、吳旭豐、陳佳欣、蘇詠嵐、湯佩樺、陳櫛昕、李孟儒、曾鈺純、陳宜妙、鄭安洲、陳思蓉、蔡明庭、黃士峯、王翊豪、劉嘉倫、彭康禹、蔡孟真、陳玟卉、林明潔、陳怡潔、王奕勛、胡振豪、許文苑、高紹恩、陳彥廷、蔡岱蓁、巫佳儒、吳雨潔、蔡佩娟、吳思葶、劉采蓉、賴駿琦、賴怡瑄、金麟娟、陳迦燈、蔡佳穎

Alumni:( 研究助理)
: 廖偉莉、吳其真、邱上頤、謝佩珍、林宜玲、黃雅芳、鍾威廉、林秋宏、許嘉伊、 廖梅真、卓逸明、洪子茗、巫毓潔、郭則君、呂良穗、張鈺媖、王淑明、陳楚穎、曾薇洲、王振宇、江玉雯、林蔚婷、劉昱攢、游淑婷、陳于榛、饒益敏

Original reports: (* corresponding author):

  1. Lee F.-J. S. and Hassan H. M. (1985). "Biosynthesis of Superoxide Dismutase in Saccharomyces cerevisiae: Effects of Paraquat and Copper" J. Free Radicals in Biology and Medicine. 1: 319-325. 
  2. Lee F.-J. S. and Hassan H. M.  (1986). "Biosynthesis of Superoxide Dismutase and Catalase in Saccharomyces cerevisiae: Effects of Oxygen and Cytochrome-c Deficiency" J.  Industrial Microbiol. 1: 187-193. 
  3. Lee F.-J. S. and Hassan H. M.  (1987). "Biosynthesis of Superoxide Dismutase and Catalase in Chemostat Culture of Saccharomyces cerevisiae" Appl. Microbiol. and Biotech. 26: 531-536.
  4. Lee F.-J. S. and Hassan H. M. (1987). "Effect of Oxygen Tension on Expression and Stability of a Killer Toxin Chimeric Plasmid in Chemostat Culture of Saccharomyces cerevisiae" Appl. Microbiol. and Biotech. 27: 72-74.
  5. Lee F.-J. S. and Hassan H. M. (1988). "Stability and Expression of a Plasmid Containing Killer Toxin cDNA in Batch and Chemostat Cultures of Saccharomyces cerevisiae" Biotech. and Bioeng. 31: 783-789.
  6. Lee F.-J. S., Lin L.-W., and Smith J. A. (1988). "Purification and Characterization of an Na-Acetyltransferase from Saccharomyces cerevisiae" J. Biol. Chem. 263: 14948- 14955.
  7. Hassan H. M. and Lee F.-J. S. (1989). "Effect of Temperature and htpR on the Biosynthesis of Superoxide Dismutase in Escherichia coli" FEMS Microbiol. Let. 58:133-138.
  8. Lin L.-W., Lee F.-J. S., and Smith J. A. (1989). "Structural Organization of The Rat Acyl-Peptide Hydrolase Gene" Nucleic Acids Research 17: 4397-4400.
  9. Lee F.-J. S., Lin L.-W., and Smith J. A. (1989). "Molecular Cloning and Sequencing of a cDNA Encoding Na-Acetyltransferase from Saccharomyces cerevisiae" J. Biol. Chem. 264: 12339-12343.
  10. Lee F.-J. S., Lin L.-W., and Smith J. A. (1989). "Purification and Characterization of an Acetyl-CoA Hydrolase from Saccharomyces cerevisiae" Eur. J. Biochem. 184: 21-28.
  11. Lee F.-J. S., Lin L.-W., and Smith J. A. (1989). "Na-Acetyltion is Required for Normal Growth and Mating in Saccharomyces cerevisiae" J. Bacteriol. 171: 5795-5802.
  12. Lee F.-J. S., Lin L.-W., and Smith J. A. (1989). "Na-Acetyltransferase Deficiency Alters Protein Synthesis in Saccharomyces cerevisiae" FEBS Lett. 256: 139-142.
  13. Lee F.-J. S., Lin L.-W., and Smith J. A. (1990). "Identification of a Methionine-Specific-Na-Acetyltransferase in Saccharomyces cerevisiae" J. Biol. Chem. 265: 3603-3606.
  14. Lee F.-J. S., Lin L.-W., and Smith J. A. (1990). "A Glucose-repressible Gene Encodes Acetyl-CoA Hydrolase from Saccharomyces cerevisiae" J. Biol. Chem. 265: 7413-7418.
  15. Lee F.-J. S., Lin L.-W., and Smith J. A. (1990). "Model Peptides Reveal Specificity of Na-Acetyltransferase from Saccharomyces cerevisiae" J. Biol. Chem. 265: 11576-11580.
  16. *Lee F.-J. S. and Moss J. (1991) "Cloning of a Saccharomyces cerevisiae Gene Encoding a Protein Homologous to Allantoicase of Neurospora crassa" Yeast 7: 993-995.
  17. *Lee F.-J. S. (1992). "Modified Protocol for Yeast DNA Mini-preparation" BioTechniques     12: 677.
  18. Murtagh J. J., Mowatt M. R., Lee C.-M., Lee F.-J. S., Mishima K., Nash T. E., Moss J., and Vaughan M. (1992). "Guanine Nucleotide-Binding Proteins in the Intestinal Parasite Giardia lamblia: Isolation of a Gene Encoding a ~20 kDa ADP-Ribosylation Factor" J. Biol. Chem. 267: 9654-9662.
  19. *Lee F.-J. S., Moss J., and Vaughan M. (1992). "Human and Giardia ADP-Ribosylation Factors (ARFs) Complement ARF Function in Saccharomyces cerevisiae" J. Biol. Chem. 267: 24441-24445.
  20. *Lee F.-J. S., Moss J., and Lin L. W. (1992). "A Simplified Hybridization Protocol for RNA Blot" BioTechniques 13: 844-845.
  21. Murtagh J. J., Lee F.-J. S., Deak P., Hall L. M., Monaco L., Lee C.-M., Stevens L. A., Moss J., and Vaughan M. (1993). "Molecular Characterization of a Conserved, Guanine Nucleotide-Dependent ADP-ribosylation Factor in Drosophila melanogaster" Biochemistry 32: 6011-6018.
  22. *Lee F.-J. S. and Moss J. (1993). "A RNA-Binding Protein Gene (RBP1) of Saccharomyces cerevisiae Encodes a Putative Glucose Repressible Protein Containing Two RNA Recognition Motifs" J. Biol. Chem. 268: 15080-15087.
  23. *Lee F.-J. S., Stevens L. A., Kao Y. L., Moss J., and Vaughan M. (1994). "Characterization of a Glucose-Repressible Yeast ADP-ribosylation Factor 3 (ARF3) from Saccharomyces cerevisiae" J. Biol. Chem. 269: 20931-20937.
  24. *Lee F.-J. S., Stevens L. A., Hall L. M., Murtagh J. J., Kao Y. L., Moss J., and Vaughan M. (1994). "Characterization of Class II and Class III ADP-ribosylation Factor Genes and Proteins in Drosophila melanogaster" J. Biol. Chem. 269: 21555-21560.
  25. Zhang G.-F., Patton W.A., Lee F.-J. S., Liyanage M., Han J.S., Rhee S.G., Moss J., and Vaughan M. (1995). "Different ARF Domains Are Required for the Activation of Cholera Toxin and Phospholipase D" J. Biol. Chem. 270: 21-24.
  26. *Lee F.-J. S., Lin L.-W., and Smith J. A. (1996)."Acetyl-CoA Hydrolase Involved in Acetate Utilization in Saccharomyces cerevisiae" Biochimica et Biophysica Acta, 1297: 105-109.
  27. *Lee F.-J. S., Lin L.-W., and Smith J. A. (1997). "A Na‑Acetyltransferase Selectively Transfers an Acetyl group to NH2-terminal Methionine Residues: purification and partial characterization" Biochimica et Biophysica Acta, 1338: 244-252.
  28. Lee F.-J. S., Patton W., Lin C.-Y., Moss J, Vaughan M, Goldman N. D., and Syin S. (1997). "Identification and Characterization of an ADP-ribosylation Factor in Plasmodium falciparum " Mol. Biochem. Parasitol. 87: 217-223.
  29. *Lee F.-J. S., Huang C.-F., Yu W.-L., Buu L.-M., Lin C.-Y., Huang M.-C., Moss, J., and Vaughan, M. (1997). "Characterization of an ADP-ribosylation Factor-like 1 Protein in Saccharomyces cerevisiae" J. Biol. Chem. 272:30998-31005.
  30. Hong J-X., *Lee F-J. S., Patton W.A., Lin C.-Y., Moss J., and Vaughan M. (1998)."Phospholipid- and GTP- dependent Activation of Cholera Toxin and Phospholipase D by Human ADP-Ribosylation Factor-like Protein 1 (HARL1)" J. Biol. Chem. 273:15872- 15876.
  31. Wu C.H, Tsai-Wu J.-J., Huang Y.-T., Lin C.-Y. , Liou G.-G., and *Lee F.-J. S. (1998). "Identification and Subcellular Localization of a Novel Cu,Zn Superoxide Dismutase of Mycobacterium tuberculosis" FEBS Lett. 439:192-196.
  32. Huang C.-F., Buu L.-M., Yu W.-L., and *Lee F.-J. S. (1999). "Characterization of a Novel ADP-Ribosylation Factor-like Protein (yARL3) from Saccharomyces cerevisiae" J. Biol. Chem. 274:3819-3827.
  33. Pacheco-Rodriguez G., Patton W.A., Adamik R., Yoo H.S., Lee F.-J. S., Zhang G.-F. Moss J., and Vaughan M. (1999). " Structural Elements of ADP Ribosylation Factor 1 Required for Functional Interaction with Cytohesin-1" J. Biol. Chem. 274: 12438-12444.
  34. Lin C.-Y., Huang P.-H., Liao W.-L., Cheng H.-J., Huang C.-F., Kuo J.C., Patton W.A., Massenburg D., Moss J., and *Lee F.-J. S. (2000). "ARL4, an ARF-like Protein that is Developmentally Regulated and Localized to Nuclei and Nucleoli" J. Biol. Chem. 275: 37815-37823.
  35. Huang C.-F., Chen C.-C., Tung L., Buu L.-M., and *Lee F.-J. S. (2002). " The yeast ADP-ribosylation factor GAP, Gcs1, is involved in maintenance of mitochondrial morphology” J. Cell Sci. 115: 275-282.
  36. Lin C.-Y., Li C.-C., Huang P.-H., and *Lee F.-J. S. (2002). "A Developmentally Regulated ARF-like protein (ARL5), localized to Nuclei and Nucleoli, Is Interacted with Heterochromatin Protein 1" J. Cell Sci. 115: 4433-4445.
  37. Chen Y.-C, Wu C.-C., Chung W.-L., and *Lee F.-J. S. (2002). "Differential Secretion of SAP 4-6 Proteins in Candida albicans during Hypha Formation" Microbiology 148: 3743-3754.
  38. Buu L.-M., Chen Y.-C., and *Lee F.-J. S. (2003) "Functional Characterization and Localization of Acetyl-CoA Hydrolase, Ach1p, in Saccharomyces cerevisiae" J. Biol. Chem.  278: 17203-17209.
  39. Huang C.-F., Liu Y.-W., Tung L., Lin C.-H., and *Lee F.-J. S. (2003) " Role for Arf3p in Development of Polarity, but not Endocytosis, in Saccharomyces cerevisiae " Mol. Biol. Cell 14: 3834-3847.
  40. Buu L.-M., Jang L.-T., and *Lee F.-J. S. (2004) "The Yeast RNA-binding Protein Rbp1p Modifies Stability of Mitochondrial Porin mRNA" J. Biol. Chem. 279: 453-462.
  41. Yu C.-J., Chen Y.-C., Hsiao C.-H., Kuo T.-C., Chang S. C., Lu C.-Y., Wei W.-C., Lee C.-H.,  Huang L. M., Chang M. F., Ho H.-N, and *Lee F.-J. S. (2004) "Identification of a Novel Protein 3a from Severe Acute Respiratory Syndrome Coronavirus" FEBS Lett. 565: 111-116. (Has been cited for 44 times)
  42. Liu Y.-W., Huang C.-F., Huang, K.-B., and *Lee F.-J. S. (2005) "Role for Gcs1p in Regulation of Arl1p at Trans-Golgi Compartments " Mol. Biol. Cell 16: 4024-4033. (Selected in the “Faculty of 1000 Biology”)
  43. Liu Y.-W., Lee S.-W., and *Lee F.-J. S. (2006) "Arl1p is Involved in Transport the GPI-anchored Protein Gas1p from the Late Golgi to the Plasma Membrane" J. Cell Sci. 119: 3845-3855. (Highlighted in the “In this Issue”)
  44. Jang L.-T., Buu L.-M., and *Lee F.-J. S. (2006) " Determinants of Rbp1p Localization in Specific Cytoplasmic mRNA-processing Foci, P-bodies" J. Biol. Chem. 281: 29379-29390.
  45. Li C.-C., Chiang, T.-C., Wu T.-S., Pacheco-Rodriguez G., Moss J., and *Lee F.-J. S. (2007) "ARL4D Recruits Cytohesin-2/ARNO to Modulate Actin Remodeling" Mol. Biol. Cell 18: 4420-4437.
  46. Tsai P.-C., Lee S.-W., Liu Y.-W., Chu C.-W., Chen K.-Y., Ho J.-C., and *Lee F.-J. S. (2008) "Afi1p Functions as an Arf3p Polarization-Specific Docking Factor for Development of Polarity" J. Biol. Chem. 283: 16915-16927.
  47. Chen K.-Y., Tsai P.-C., Hsu J.-W., Hsu H.-C., Fang C.-Y., Chang L.-C., Tsai Y.-T, Yu C.-J., and *Lee F.-J. S. (2010) "Syt1p Promotes Activation of Arl1p at the Late Golgi to Recruit Imh1p" J. Cell Sci. 123: 3478-3489. (Highlighted in the “In this Issue”)
  48. Lin Y.-C., Chiang, T.-C., Liu, Y.-T., Tsai, Y.-T., Jang, L.-T., and *Lee F.-J. S. (2011) " ARL4A Acts with GCC185 to Modulate Golgi Complex Organization" J. Cell Sci. 124: 4014-4026.
  49. Lu J.-Y., Lin Y.-Y., Sheu J.-C., Wu J.-T., Lee F.-J., Chen Y., Lin M.-I, Chiang F.-T., Tai T.- Y., Berger S. L., Tsai K.-S., Zhu H., Chuang L.-M., Boeke J. D.  (2011) "Acetylation of Yeast AMPK Controls Intrinsic Aging Independently of Caloric Restriction" Cell 146: 969-979.
  50. Patel M., Chiang T.-C., Tran V., *Lee F.-J. S., and *Côté J.-F. (2011) "The Arf family GTPase, Arl4A, Complexes with ELMO to Promote Actin Cytoskeleton Remodeling and Reveals a Versatile Ras-Binding Domain in the ELMO Protein Family" J. Biol. Chem. 286: 38969-38979. (* Co-corresponding author)
  51. Chang L.-C., and *Lee F.-J. S. (2012) "The RNA helicase Dhh1p Cooperates with Rbp1p to Promote Porin mRNA Decay via its Nonconserved C-terminal Domain" Nucleic Acids Res. 40: 1331-1344.
  52. Chen K.-Y., Tsai P.-C., Liu Y.-W., and *Lee F.-J. S. (2012) "Competition between the golgin Imh1p and the Gcs1p GAP stabilizes activated Arl1p at the late-Golgi" J. Cell Sci. 125: 4586-4596.
  53. Li C.-C., Wu T.-S., Huang C.-F., Jang L.-T., Liu Y.-T., You S.-T., Liou G.-G., and *Lee F.-J. S. (2012) "GTP-binding-defective ARL4D alters mitochondrial morphology and membrane potential" PLoS ONE 7(8): e43552.
  54. Chen P.-H., Chien F.-C., Lee S.-P., Chan W.-E., Lin I-H., Liu C.-S., Lee F.-J., Lai J.-S., Chen P., Yang-Yen H.-F. and Yen J. J.-Y.  (2012) "Identification of a novel function of the clathrin-coated structure at the plasma membrane in facilitating GM-CSF receptor-mediated activation of JAK2" Cell Cycle. 11: 3611-3626.
  55. Tsai P.-C., Hsu J.-W., Liu Y.-W., Chen K.-Y., and *Lee F.-J. S. (2013) "Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p" Proc. Natl. Acad. Sci. U S A. 110: E613-622 (Selected in “From the Cover” with a Commentary on page 2691), (Recommended as being of special significance in the “Faculty of 1000”)
  56. Chiang Y-J, Ho K-C, Sun C-T, Chiu J-J, Lee F-J, Liao F., Yang-Yen H.-F. and Yen J. J.-Y. (2013) CBAP Functions as a Novel Component in Chemokine-Induced ZAP70-Mediated T-Cell Adhesion and Migration. PLoS ONE 8(4): e61761.
  57. Hsu J.-W. and *Lee F.-J. S. (2013) "Arf3p GTPase is a key regulator of Bud2p activation for invasive growth in Saccharomyces cerevisiae" Mol. Biol. Cell 24: 2328-2339.
  58. Hsu J.-W., Chang L.-C., Jang L.-T., Huang C.-F., and *Lee F.-J. S. (2013) "The N-terminus of Vps74p is essential for the retention of glycosyltransferases in the Golgi but not for the modulation of apical polarized growth in Saccharomyces cerevisiae" PLoS ONE 8(9): e74715.
  59. Hsu J.-W., Chen Z.-J., Liu Y.-W., and *Lee F.-J. S. (2014) "Mechanism of action of flippase Drs2p in modulating GTP hydrolysis of Arl1p" J. Cell Sci. 127: 2615-2620.
  60. Hsu J.-W., Chen K-J., and *Lee F.-J. S. (2015) "Snf1/AMP-activated protein kinase activates Arf3p to promote invasive yeast growth via a non-canonical GEF domain" Nature Communs 6:7840/DOI:10.1038/ncomms8840.
  61. Hsu J.-W., Tang P.-H., Wang I-H., Liu C.-L., Chen W.-H., Tsai P.-C., Chen K.-Y., Chen K.-J., Yu C.-J., and *Lee F.-J. S. (2016) "Unfolded protein response regulates yeast Arl1p activation at late-Golgi via phosphorylation of Arf GEF Syt1p" Proc. Natl. Acad. Sci. U S A. 113: E1683-E1690.
  62. Wang I-H., Chen Y-J., Hsu J.-W., and *Lee F.-J. S. (2017) "The Arl3 and Arl1 GTPase cooperates with Cog8 to regulate selective autophagy via Atg9 trafficking" Traffic 18:580-589.
  63. Chiang T.-S., Wu H.-F., and *Lee F.-J. S. (2017) "ADP-ribosylation factor-like 4C binding to Filamin-A modulates filopodium formation and cell migration" Mol. Biol. Cell 28: 3013-3028.
  64. Chiang T.-S., Lin M.-C., Tsai M.-C., Chen C.-H, Jang L.-T., and *Lee F.-J. S. (2019) " ADP-ribosylation factor-like 4A interacts with Robo1 to promote cell migration via regulating Cdc42 activation" Mol. Biol. Cell 30: 69-81.
  65. Chen Y.-T., Wang I.-H., Wang Y.-H., Chiu W.-Y., Hu J.-H., Chen W.-H., and *Lee F.-J. S. (2019) "Action of Arl1 GTPase and golgin Imh1 in Ypt6-independent retrograde transport from endosomes to the trans-Golgi network" Mol. Biol. Cell 30: 1008-1019.
  66. Chen K.-J., Chiang T.-C., Yu C.-J., and *Lee F.-J. S. (2020) "Cooperative recruitment of Arl4A and Pak1 to the plasma membrane contributes to sustained Pak1 activation for cell migration" J. Cell Sci.  133: jcs233361. doi:10.1242/jcs233361
  67. Lin S.-J., Huang C.-F., Wu T.-S., Li C.-C, and *Lee F.-J. S. (2020) "Arl4D-EB1 interaction promotes centrosomal recruitment of EB1 and microtubule growth" Mol. Biol. Cell 31: 2348-2362.
  68. Wang Y.-H., Chiu W.Y., Chen Y.-T., Cai P.-J., Wu Y.-C., Wu J.-L, Chen B. H., Liu Y.-W., Yu C.-J., and *Lee F.-J. S.(2022) "Golgin Imh1 and GARP complex cooperate to restore the impaired SNARE recycling transport induced by ER stress" Cell Reports 38: 110488
  69. Lin M.-C., Yu C.-J., and *Lee F.-J. S. (2022) " Phosphorylation of Arl4A/D promotes their binding by the HYPK chaperone for their stable recruitment to the plasma membrane " Proc. Natl. Acad. Sci. U S A.119 (30) e2207414119
  70. Chen K.-J., Hsu J.-W., and *Lee F.-J. S. (2022) "AMPK promotes Arf6 activation in a kinase-independent manner upon glucose starvation" J. Cell Sci. 135: jcs259609. (Selected in the “RESEARCH HIGHLIGHTS”, e135_e1801)
  71. Lai C.-C., Chiu W.-Y., Chen Y.-T., Wu C.-L. and *Lee F.-J. S. (2023) "The SNARE- associated protein Sft2 functions in Imh1-mediated SNARE recycling transport upon ER stress" Mol. Biol. Cell, 34: ar112, 1-15. (Selected in ASCB JOURNAL NEWS)
  72. Lin S.-J., Liu T.-J., Tsai Y.-T., Tsai M.-T. and *Lee F.-J. S. (2023) "Endosomal Arl4A attenuates EGFR degradation by delaying its deubiquitination at the late endosome" Nature Communs. DOI 10.1038/s41467-023-42979-9 (NHRI funded)

Reviews:

  1. Hassan H. M. and Lee F.-J. S. (1986). "Effects of Cytochrome-c Deficiency, Paraquat, and Copper on the Biosynthesis of Copper-Zinc Superoxide Dismutase in Saccharomyces cerevisiae" In Superoxide and Superoxide Dismutase in Chemistry, Biology and Medicine, Elsevier Sci. Publisher., p. 300-303.
  2. Lee F.-J. S., Lin L.-W., and Smith J. A.  (1988). "Amino-Terminal Processing of Eukaryotic     Proteins by N-Acetylation: Substrate Specificity of Yeast Na-Acetyltransferase" In         Advances in Gene Technology: Protein Engineering and Production. ISCU Short Report.    Miami: IRL Press Ltd. 8:36
  3. Lee F.-J. S., Lin L.-W., and Smith J. A. (1989). "Na-Acetylation of Eukaryotic Proteins: Purification and Characterization of Yeast Na-Acetyltransferase and Acetyl Coenzyme A Hydrolase" In: Wittmann-Liebold B, ed, Methods in Protein Sequence Analysis, Amsterdam: Elsevier Sci. Publisher, p.352-255.
  4. Smith J. A., and Lee F.-J. S. (1989). "Model Peptides Reveal Specificity of Molecular Recognition for Acetylation by Na-Acetyltransferase" Rivier JE, Marshall GR eds. Peptides. Leiden NV:Escom Science Publishers, p. 485-488.
  5. Smith J. A., Lee F.-J. S., and Lin L.-W. (1990). "Na-Acetylation of Eukaryotic Proteins is         Regulated by Two Na-Acetyltransferases" J. Protein Chem. 9: 302-303
  6. Smith J. A., Lee F.-J. S., and Lin L.-W. (1993). "NH2-terminal Processing of Eukaryotic   Proteins" ABRF News 4: 12-13
  7. Moss J., Haun RS., Tsai SC., Welsh CF., Lee F.-J.S., Price SR., and Vaughan, M.      (1994) "Activation of cholera toxin by ADP-ribosylation factors: 20-kDa guanine nucleotide-binding proteins". Methods Enzymol. 237:44-63.
  8. *Lee F.-J.S., and Huang C.-F. (2001) "Purification, Properties and Analysis of yARL3". Methods Enzymol. 329: 417-423.
  9. Li C.-C., and *Lee F.-J. S. (2010) "Arf-like protein 4D" UCSD-Nature Molecule Pages
. 19 Feb 2010 | doi:10.1038/mp. a003973.01
  10. Yu C.-J, and *Lee F.-J. S. (2017) "Multiple activities of Arl1 GTPase in the trans-Golgi network" J. Cell Sci. 130: 1691-1699.
  11. Sztul E., Chen P.-W., Casanova J. E., Cherfils J., Dacks J. B., Lambright D. G., Lee F.-J.S., Randazzo P. A., Santy L. C., Schürmann A., Wilhelmi I., Yohe M. E., and Kahn R. A. (2019) "ARF GTPases and their GEFs and GAPs: concepts and challenges" Mol. Biol. Cell 30: 1249-1271.

  1. Smith J. A. and Lee F.-J. S. "Isolation, Purification, Characterization, Cloning and Sequencing of Na-Acetyltransferase"
    U.S. Patent No. 4,966,848 (October 30, 1990); U.S. Patent No. 5,128,459 (July 7, 1992)
    U.S. Patent No. 5, 283,188 (February 1, 1994)
  2. Smith J. A. and Lee F.-J. S. "Isolation of Strains of Saccharomyces cerevisiae Having Altered Alpha-Acetyltransferase activity"
    U.S. Patent No. 5,210,024 (May 11, 1993) 
  3. Smith J. A. and Lee F.-J. S. "Identification of Methionine N-Acetyltransferase"
    USA Patent No.5,223,421 (June 29, 1993) 
  4. Smith J. A., Lee F.-J. S., and Lin L.-W. "Glucose-Regulated Promoter of Yeast Acetyl-CoA Hydrolase".
    "Isolation, Purification, Characterization, Cloning and Sequencing of Acetyl-CoA Hydrolase"
    European Patent No. 93111752.7; USA Patent No.5,487,990 (June 30, 1996) 
  5. Lee F.-J. S. and Huang C.-F. "Identification of an ADP-Ribosylation Factor-like Protein from Saccharomyces cerevisiae"
    USA Patent No. 6,562,614 B2 (May 13, 2003) 
  6. Lee F.-J. S. and Wu C.H. "Identification of a novel Cu,Zn superoxide dismutase of Mycobacterium tuberculosis "
    USA Patent No. 6,517,845 (February 11, 2003)
    USA Patent No. 7,094,403 (August 22, 2006) 
    P.R.C. Patent No. ZL99815528.4 (May 21, 2008)
  7. Lee F.-J. S. , Yu C.-J., Chang M. F., and Ho H.-N "Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus" USA Patent No. 7,691,309 B2 (April 6, 2010)