劉雅雯

劉雅雯 Ph.D. Ya-Wen Liu

專任副教授 Associate Professor

研究領域:
膜動態的調控
membrane dynamics

研 究 室:
基礎醫學大樓1517室
Basic Medicine Building 1517

Tel:886-2-23123456 ext. 88586 (office) or 88587 (lab)

  1. Regulation of Dynamin-mediated Membrane Division

Hyperactive, disease-associated Dynamin-2 mutation results in T-tubule fragmentation and muscle atrophy of Drosophila. So, we would like to know how exactly Dynamin-2 activity is regulated in muscle cells.               

                                                                                     

  1. Function of Podosome in Muscle Cells


Podosome is a multipurpose organelle existing in different kinds of cells. In muscle cells, podosome participates in several differentiation processes, including myoblast fusion and neuromuscular junction development. We are currently studying the function of Dynamin-2 on podosome activity regulation.

 

  1. Functions of Phospholipase D (PLDs) on Membrane Remodeling


PLDs are conserved and critical phosphatidic acid producing enzyme that is a critical signaling lipid. We are currently study the activity PLDs and its downstream effectors in membrane remodeling processes.

Sep 2000-Jun 2006
Ph.D. /  Institute of Molecular Medicine, Master National Taiwan University .
Thesis: Functional Characterization of Arl1p in yeast Saccharomyces cerevisiae.
 

Sep 1996-Jun 2000
B.S. / Department of Biology, National Cheng Kong University

2020~
Associate Professor
National Taiwan University, Institute of Molecular Medicine

 

2014-2020
Assistant Professor
National Taiwan University, Institute of Molecular Medicine

 

2012-2014
Visiting scholar
National Taiwan University, Institute of Molecular Medicine

 

2007-2012
Postdoctoral Fellow
The Scripps Research Institute, Department of Cell Biology

 

2006-2007
Postdoctoral Fellow
National Taiwan University, Institute of Molecular Medicine

 

Video: A Day in Lab

 

 

劉雅雯 Ya-Wen Liu (PI)

 

CURRENT MEMBERS

  • 莊梅均 Mei-Chun Chuang (Maggie)
    Postdoctoral fellow
    Hometown: Kaohsiung, Taiwan
    Education:
    B.S., Department of Bioagricultural Science and Technology, National Chia-Yi University
    Ph.D., Institute of Molecular Medicine, National Taiwan University .
  • 林珊珊 Shan-Shan Lin (Olga)
    Postdoctoral fellow
    Hometown: Taipei, Taiwan
    Education: B.S., Department of Medical Biotechnology and Laboratory Science, Chang Gung University
  • 黎嘉雯 Jessica Laiman,
    PhD Student, Class 2016
    Hometown: Jakarta, Indonesia
    Education: B.S., Department of Life Sciences, National Taiwan Normal University
  • 蘇佑安 You-An Su (Sandy)
    PhD Student, Class 2019
    Hometown: Taipei, Taiwan
    Education: B.S., Department of Bioscience and Biotechnology, National Taiwan Ocean University
  • 王貞 Chen Wang (Jane)
    PhD Student, Class 2020
    Hometown: Taipei, Taiwan
    Education: 
    B.S., Department of Life Sciences, National Tsing Hua University
    M.S., Institute of Molecular Medicine, National Tsing Hua University
  • 邱欣儀 Hsin-Yi Chiu (Carol)
    Master Student, Class 2019
    Hometown: Hsinchu, Taiwan
    Education: B.S., Department of medical biotechnology and laboratory science, Chang Gung University

LAB ALUMNI

  • 李昱賢 Yu-Hsien Lee (Albert)
    Undergraduate (2013)
    Hometown: Taipei, Taiwan
    Education: BS, Department of Chemistry, National Taiwan University
  • 金鈺頷 Yu-Han Jin
    Master Student, Class 2013 (co-advisor: Dr. June-Tai Wu)
    Hometown: Changhua, Taiwan
    Education: BS, Department of Biochemical Science and Technology, National Taiwan University
  • 駱怡君 Julie Loh
    Master Student, Class 2016
    Hometown: Bothell, Washington, United States
    Education: B.S. Physiology, B.A. Psychology, University of Washington
  • 張瑜真 Joyce Chang
    Master Student, Class 2017
    Hometown: Taipei, Taiwan
    Education: B.S., Department of Life Sciences, National Tsing Hua University
  • 謝宗霖 Tsung-Lin Hsieh (Cliff)
    Master Student, Class 2018
    Hometown: Hsinchu, Taiwan
    Education: B.S., Department of medical biotechnology and laboratory science, Taipei Medical University

  1. Lin, S.S., Hsieh, T.L., Liou, G.G., Li, T.N., Lin, H.C., Chang, C.W., Wu, H.Y., Yao, C.K., and Liu, Y.W. * (2020) Dynamin-2 regulates postsynaptic cytoskeleton organization and neuromuscular junction development. Cell Rep. 33(4):108310.
  2. Laimann, J. and Liu, Y.W.* (2020) Cellular assays for measuring dynamin activity in muscle cells. Methods in Molecular Biology, 2159:179-187.
  3. Julie, L.#, Chuang, M.C.#, Lin, S.S.#, Joseph, J., Su, Y.A., Hsieh, T.L. Chang, Y.C., Liu, A.P. and Liu, Y.W.* (2019). An Acute Decrease in Plasma Membrane Tension Induces Macropinocytosis via PLD2 Activation. Journal of Cell Science, 132 (17). doi: 10.1242/jcs.232579. (selected as Research Highlight)
  4. Chuang, M.C., Lin, S.S., Ohniwa, R.L., Lee, G.H., Su, Y.A., Chang, Y.C., Tang, M.J. and Liu, Y.W. * (2019). Tks5 and Dynamin-2 enhance actin bundle rigidity in invadosomes to promote myoblast fusion. Journal of Cell Biology. 218(5), 1670-85.
  5. Lin, S.S. and Liu, Y.W. * (2019). Mechanical stretch induces mTOR recruitment and activation at the phosphatidic acid-enriched macropinosome in muscle cell. Front Cell Dev Biol. doi: 10.3389/fcell.2019.00078.
  6. Cheng, A.M.S., Yin, H.Y., Chen A., Liu, Y.W., Chuang, M.C., He, H., Tighe, S., Sheha,. H., Liao, S.L. * (2016). Celecoxib and pioglitazone as potential therapeutics for regulating TGF-β-induced hyaluronan in dysthyroid myopathy. Investigative Opthalmology & Visial Science, 57(4),1951-1959.
  7. Chin, Y.H.*, Lee, A.*, Kan, H.W., Laiman J., Chuang, M.C., Hsieh, S.T. and Liu, Y.W. * (2015), Dynamin-2 mutations associated with centronuclear myopathy are hypermorphic and lead to T-tubule fragmentation. Hum Mol Genet 24, 5542-54 (* equal contribution).
  8. Chen, C.H., Lee, A., Liao, C.P., Liu, Y.W., and Pan, C.L. * (2014). RHGF-1/PDZ-RhoGEF and retrograde DLK-1 signaling drive neuronal remodeling on microtubule disassembly. Proc Natl Acad Sci USA, 111(46):16568-73.
  9. Hsu, J.W., Chen, Z.J., Liu, Y.W. and Lee, F.J. * (2014). Mechanism of action of the flippase Drs2p in modulating GTP hydrolysis of Arl1p. Journal of Cell Science, 127(Pt 12):2615-20.
  10. Liu, Y.W., Mattila, J.P. and Schmid, S.L. (2013), Dynamin-catalyzed membrane fission requires coordinated GTP hydrolysis. PLOS ONE 8, e55691.
  11. Tsai, P.C., Hsu, J.W., Liu, Y.W., Chen, K.Y. and Lee, F.J. * (2013), Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p. Proc Natl Acad Sci USA 110, E668-77 
  12. Chen, K.Y., Tsai, P.C., Liu, Y.W. and Lee, F.J. * (2012), Competition between the golgin Imh1p and the Gcs1p GAP stabilizes activated Arl1p at the late-Golgi. J. Cell Sci. 125, 4586-96. 
  13. Liu, Y.W., Su, A.I. and Schmid, S.L* (2012), The evolution of dynamin to regulate clathrin-mediated endocytosis. BioEssays 34, 643-7.
  14. Liu, Y.W., Lukiyanchuk, V., and Schmid, S.L. * (2011), Common membrane trafficking defects of disease-associated dynamin 2 mutations. Traffic 12, 1620-33.
  15. Liu, Y.W.*, Neumann, N.*, Ramachandran, R., Ferguson, S.M., Pucadyil, T.J., and Schmid, S.L. (2011), Differential curvature sensing and generating activities of dynamin isoforms provide opportunities for tissue-specific regulation. Proc Natl Acad Sci USA 108, 234-42. (* equal contribution; selected and evaluated at “Faculty of 1000”.)
  16. Ramachandran, R., Pucadyil, T.J., Liu, Y.W., Acharya, S., Leonard, M., Lukiyanchuk, V., and Schmid, S.L. * (2009), Membrane insertion of the pleckstrin homology domain variable loop 1 is critical for dynamin-catalyzed vesicle scission. Mol. Biol. Cell. 20:4630-9.
  17. Chappie, J.S., Acharya, S., Liu, Y.W., Leonard, M., Pucadyil, T.J., and Schmid, S.L. * (2009), An intramolecular signaling element that modulates dynamin function in vitro and in vivo. Mol. Biol. Cell. 20: 3561-71.
  18. Liu, Y.W., Surka, M.C., Schroeter, T., Lukiyanchuk, V., and Schmid, S.L. * (2008), Isoform and splice-variant specific functions of dynamin-2 revealed by analysis of conditional knock-out cells. Mol. Biol. Cell. 19: 5347-59. (selected for “InCytes of MBC”)
  19. Tsai, P.C. *, Lee, S.W. *, Liu, Y.W.*, Chu, C.W., Chen, K.Y., Ho, J.C., and Lee, F.J. * (2008), Afi1p functions as an Arf3p polarization-specific docking factor for development of polarity. J Biol Chem. 283: 16915-27. (* equal contribution.)
  20. Liu, Y.W., Lee, S. W., and Lee, F.J. * (2006), Arl1p is involved in transport of the GPI-anchored protein Gas1p from the late Golgi to the plasma membrane. J. Cell Sci. 119: 3845-55. (highlighted in the “IN this Issue”)
  21. Liu, Y.W., Huang, C.F., Huang, K.B., and Lee, F.J. * (2005), Role for Gcs1p in regulation of Arl1p at trans-Golgi compartments. Mol. Biol. Cell. 16: 4024-33. (selected in the “Faculty of 1000”)
  22. Huang, C. F. *, Liu, Y.W. *, Tung, L., Lin, C.H., and Lee, F.J. * (2003), Role for Arf3p in development of polarity, but not endocytosis, in Saccharomyces cerevisiae. Mol. Biol. Cell. 14: 3834-47. (* equal contribution.)